
Removed all duplicate recognition sites leaving 465 distinct recognition sequences ( my fault for not removing them in the first instance).Expanded dataset to include new recognition sites from the resource that 96well linked to.H0 rejected with 95% confidence (indeed with 99.9%+ confidence)Ĭan anyone explain or suggest why it is more common that restriction enzymes recognition sites have an even number of bases? Updates P=0.05, 1 Degree of Freedom: Critical Value of 3.841 There is no significant difference between the number of restriction sequences The results are probably best summarised graphically:

I wondered if this was just a co-incidence, so I took the data from this site for over a thousand known recognition sites and put it into a spreadsheet ( XLS uploaded here). When reading my textbook I noticed that in all examples but one from eight the recognition site was an even number of bases.
